What is radioallergosorbent test?
Radioallergosorbent test (REST test) is a method used to detect allergen specific IgE in your blood. It determines particularly the substances causing allergic response. In general, this is quite different from allergy skin test. This is because, allergy skin test determines allergy the reaction from an individual’s skin to different substances. But radioallergosorbent test checks allergic sensitivity, done on a sample of blood. According to internal medicine and otolaryngology, REST is a recognized and effective test for allergy.
How Radioallergosorbent test detecting your allergic sensitivity?
Radioallergosorbent test detect allergen specific IgE antibodies, which is responsible for hypersensitivity. Hypersensitivity is a reaction or intolerance produced by undesirable reactions of normal immune system, including allergies and autoimmunity. These allergens bound to patient’s serum and insoluble material, reacted by following conjugations. Allergen will form complex, if the serum contains specific antibody. After that, radiolabeled anti-human IgE antibody is added the place where it will bind with the specific IgE. IgE serum is proposal to the amount of radioactivity.
Radioallergosorbent test are often performed with skin tests. It is also used in the situations where other tests considered to be risky. For example, severe allergic reaction experienced by a person after eating some type of foods. Main reason, the test performed by drawing blood samples, not in the patient’s skin or body. So there is minimum risk of adverse reactions taking place with prick tests or food challenges. Most likely, a lab technicians adds the allergen, to patient’s blood, bonded to a solid polymers. IgE binds to the allergen in allergic reactions, is slightly different types that that binds to each allergen.
If the patient’s immune system showing allergic to the tested allergen, then allergen-specific IgE will bind to other IgE. At that time, any other IgE will float freely in the blood. Then blood will be washed away, only leaving allergen-specific IgE. Serum sample of radioactive anti-IgE, will derived from patient who are known to be allergic to the allergen. After that, lab technician will prepare test report by determining the amount of allergen-specific IgE in the blood.